Plant-Fungus Interactions and Remediation Team (IPCR)

Research area:

The Plant-Fungus and Remediation team is specifically involved in biochemistry and microbial ecology complementary approaches. We also perform studies focusing on obligate biotrophic fungi and lipid metabolism involved in plant-fungi interactions.illustration-thematiques-ipcr-anglais-3.jpg

Assessing and exploiting soil fungi biodiversity in order to perform biological degradation of persistent organic pollutants (POP) :

Our work focuses on the practical use of saprotrophic soil fungi biodiversity in order to degrade via biological process persistent organic pollutants such as PAH or dioxins. The investigation of fungal metabolic pathways involved in their degradation (enzymatic activities, transfer and incorporation, degradation), the use of natural polysaccharides (modified starch) as POP solubilization agents and innovative methods mixing chemical (Fenton, etc …) and biological oxidation are being developed.

Use of arbuscular mycorrhizae for the phytoremediation of polluted soils and plant protection. Study of ecotoxicity of a range of pollutants (PAH, dioxins, metal trace elements and fungicides) :

The potential use of mycorrhizal symbiosis in terms of phytoremediation (biodegradation, bioaccumulation …) and of induction of plant tolerance towards stress caused by pollutants or phytopathogens is studied from the laboratory to the field scales (oxidative stress, genotoxicity, lipid metabolism). Cytological, biochemical and molecular approaches will allow elucidating corresponding involved mechanisms.

More sustainable control strategies targeting wheat fungal pathogens; induced resistance :

Two approaches aiming at reducing the use of conventionnal fungicides on wheat are performed : characterization of wheat Septoria tritici blotch (STB) populations and search for plant resistance inducers (Plant Defence Stimulators, PDS) against Blumeria graminis f.sp. tritici (powdery mildew) et Mycosphaerella graminicola (STB). Biological activities, elicitor and priming effects of these PDS, as well as those of arbuscular mycorrhizal inoculation, are evaluated at cytological, biochemical and molecular levels mainly through their impact on lipid and Reactive Oxygen Species metabolism.


Plant-Fungus Interactions and Remediation  team members:














RAFIN Catherine


RANDOUX Béatrice


REIGNAULT Philippe          

PR (responsable de l’équipe)






Collaborations :

 Regionals :

  • BioGAP, Institut Charles Viollette – ISA de Lille
  • ProBIOGEM, Institut Charles Viollette –Université de Lille
  • Laboratoire  Génie  Civil  et  géoEnvironnement, Université de Lille

Nationals :

  •  Unité de Recherche Vigne et Vin de Champagne, URCA à Reims
  • UMR Agroécologie, INRA de Dijon
  • Arvalis-Institut du Végétal

 Internationals :

  •  Laboratoire d’Amélioration Génétique des Plantes, Université d’Annaba (Algérie)
  • Université des Sciences et de la Technologie Houari Boumédiène (USTHB, Algérie)
  • Université de Djelfa
  • Helmholtz Zentrum München – German Research Centre for Environmental Health, Institute of Soil Ecology, Allemagne
  • Laboratory of Phytopathology, Université de Gand (Belgique)
  • Unité de Chimie Biologique Industrielle, Université de Liège (Gembloux Agro Bio Tech) (Belgique)
  • Laboratoire de Mycologie, Université de Louvain-la-Neuve (Belgique)
  • Agriculture et Agroalimentaire (Canada)
  • Institute for Biological Sciences (Canada)
  • Université de Wisconsin-Madison (USA)
  • Crop & Soil Systems Research Group, Scottish Agricultural College (Grande-Bretagne)
  • Faculté d’Agronomie, Université Saint-Esprit de Kaslik, université Libanaise (Liban)
  • Université Vasile Alecsandri de Bacau, Roumanie
  • Laboratoire de Génétique et Amélioration des Plantes, INAT de Tunis, Centre National de la Recherche en Agriculture Oasienne (Tunisie)
  • Centre National de la Recherche en Agriculture Oasienne (Tunisie)

PhD in progress :

Firstname Name Date of the thesis Title
Samara Mejri 2014-2017 Efficiency and plant defense stimulators modes of action on the pathosystem wheat (Triticum aestivum) – Septoria (Mycosphaerella graminicola) (ISA-Lille Financing, co-direction with Viollette Institute)
Alice Fourquez 2015-2018 Innovative solutions to health biostimulants Wheat combining applications and Toys Defense Plant (SDP) (FUI Financing IRIS + -PMCO, co-direction with Viollette Institute)
Isabelle DELSARTE 2013-2016 Development of methods of biocompatible remediation of soils polluted by dioxin, coupling a chemical oxidation process advanced to biodegradation by earth saprotrophic mushrooms. (Region-WCMP)
Imad EL ALAM 2014-2017 Characterization of pollution, toxicity and ecotoxicity of soil Bekaa in Lebanon and testing of phytoremediation by mycorrhizae. (Co-guardianship Lebanese University)
Hacène MEGLOULI 2013-2016 Evaluating the performance of mycorrhizae in phytoremediation of soils contaminated by dioxins. (DREAL)

Current research programs :


  • 2016-2018. ARIMNET – Coordination of Agricultural Research in the Mediterranean Area. BACPLANTS – Towards a sustainable agriculture by increasing plant tolerance to biotic stress under climatic change (Coordination URCA, Fr. Other partners : Institut Charles Viollette (Lille, ISA), URCA in Reims (coordinator), INRA of Marrakech and INAT of Tunis.
  •  2015-2017 : FUI IRIS+ project: «  Innovative solutions towards vinegrape’s and wheat’s health associating the application of biostimulators and Plant Defence Stimulators with imaging and agroequipment technologies » ; Other partners: INRA de Dijon, URCA, Institut Charles Viollette-ISA de Lille, Goëmar, Dijon Céréales, Tecnoma, Orange, BIBV.
  • 2016-2019. INTERREG V Fr.-Fl.-W. Portefeuille SMARTBIOCONTROL
  • 2014-2016 : NEWBIOPEST project funded by the  Nord-Pas de Calais Region: « Agricultural systems and environment in Nord-Pas de Calais » ; Other partners : Institut Charles Viollette-ProBIOGEM, Institut Charles Viollette-ISA de Lille, Pôle Légumes.
  •  Projet BIOPROTECT (Coordination PCG, Fl.) Projet BIOSCREEN (Coordination URCA, Fr.)

Recent publications:

  • Mustafa, G., Randoux, B.,  Tisserant, B., Fontaine, J., Magnin-Robert, M., Lounès-Hadj Sahraoui, A. and Reignault, Ph. (2016). Impact of phosphorus supply and plant and mycorrhizal species on the protective efficacy of arbuscular mycorrhizal fungi against wheat powdery mildew. Mycorrhiza, in press.
  • BOUCHIAT, R., VEIGNIE E., GRIZARD D., SOEBERT CH., VIGIER M., RAFIN, C. (2016). Ability of filamentous fungi to degrade four emergent water priority pollutants. Desalination and Water treatment, Article in press.
  • LENOIR I. , FONTAINE J., LOUNÈS-HADJ SAHRAOUI A. (2016) Arbuscular mycorrhizal fungal responses to abiotic stresses : a review. Phytochemistry.  123 ( 4-15).
  • LENOIR I., LOUNÈS-HADJ SAHRAOUI A., LARUELLE F., DALPE Y., FONTAINE J. (2016) Arbuscular mycorrhizal wheat inoculation promotes alkane and polycyclic aromatic hydrocarbon biodegradation: microcosm experime
  • TAYEH, CH., RANDOUX, B., TISSERANT, B., KHONG, G., JACQUES, PH., REIGNAULT, PH. (2015). Are ineffective defence reactions potential target for induced resistance during the compatible wheat-powdery mildew interaction ? Plant Physiology and Biochemistry, 18: 9-19.
  • S. FIRMIN, S. LABIDI, J. FONTAINE, F. LARUELLE, B. TISSERANT, F. NSANGANWIMANA, B. POURRUT, Y. DALPÉ, A. GRANDMOUGIN, F. DOUAY, P. SHIRALI, A. VERDIN, A. LOUNÈS-HADJ SAHRAOUI (2015) Arbuscular mycorrhizal fungal inoculation protects Miscanthus × giganteus against trace element toxicity in a highly metal-contaminated site. Science of The Total Environment 527 : 91-99.
  • F. NSANGANWIMANA, · B. POURRUT, · C. WATERLOT, · B. LOUVEL, · G. BIDAR, · S. LABIDI, · J. FONTAINE, · J. MUCHEMBLED, · A. LOUNÈS-HADJ · SAHRAOUI, · H. FOURRIER, · F DOUAY. (2015) Metal accumulation and shoot yield of Miscanthus giganteus growing in contaminated agricultural soils: Insights into agronomic practices. Agriculture Ecosystems & Environment 213 : 61-71.
  • LABIDI, S., FONTAINE, J., LARUELLE, F., TISSERANT, B., DALPE, Y., GRANDMOUGIN-FERJANI, A., DOUAY, F., LOUNES-HADJ SAHRAOUI A (2015). Fly ash-aided phytostabilisation of highly trace element polluted topsoils improves the telluric fungal biomass: A long-term field experiment. Applied Soil Ecology 85 : 69-75.
  • LABIDI S, BEN JEDDI F, TISSERANT B, YOUSFI M, SANAA M, DALPÉ Y, LOUNÈS-HADJ SAHRAOUI A (2015) Field application of mycorrhizal bio-inoculants affects the mineral uptake of a forage legume (Hedysarum coronarium L.) on a highly calcareous soil. Mycorrhiza 25 (4) :  297-309.
  • S. DRIAI, A. VERDIN, F. LARUELLE, A. BEDDIAR, A. LOUNÈS-HADJ SAHRAOUI (2015). Is the arbuscular mycorrhizal fungus Rhizophagus irregularis able to fulfil its life cycle in the presence of diesel pollution? International Biodeterioration & Biodegradation 105 : 58-65.
  • K. BENCHERIF, · A. BOUTEKRABT, · J. FONTAINE, · F. LARUELLE, · Y. DALPÈ, · A. LOUNÈS-HADJ SAHRAOUI (2015)  Impact of soil salinity on arbuscular mycorrhizal fungi biodiversity and microflora biomass associated with Tamarix articulata Vahll rhizosphere in arid and semi-arid Algerian areas. Science of The Total Environment 533 : 488-494.
  • J. MARTY, A. T. BROMAN, R. ZARNOWSKI, T. G. DWYER, L. M. BOND, A. LOUNES-HADJ SAHRAOUI, J. FONTAINE, J. M. NTAMBI, S. KELE?, CH. KENDZIORSKI, G. M. GAUTHIER. (2015). Fungal Morphology, Iron Homeostasis, and Lipid Metabolism Regulated by a GATA Transcription Factor in Blastomyces dermatitidis. PLoS Pathogens 11 (6) : e1004959.
  • Allioui N., Siah A., Brinis L., Reignault PH, Halama P. (2015). Identification of QoI-resistant genotypes of the wheat pathogen Zymoseptoria tritici in Algeria. Phytopathologia Mediterrranea, Article in press.
  •  Zarnowski R., Westler W.M., Lacmbouh G.A., Maritaj.M., Bothe J.R., Bernhardt J. , Lounes-Hadj Sahraoui A., Fontaine J., Sanchez H., Hatfield R.D., Ntambi J.M., Nett J.E., Mitchell A.P., Andes D.R. D. R. 2014. Novel entries in a fungal biofilm matrix encyclopedia. mBio, 5(4), e01333-14.
  •  Calonne M., Fontaine J., Tisserant B., Dupré de Boulois H., Grandmougin-Ferjani A., Declerck S., Lounès-Hadj Sahraoui A. 2014. Polyaromatic hydrocarbons impair phosphorus transport by the  arbuscular mycorrhizal fungus Rhizophagus irregularis. Chemosphere 104: 97-104.
  •  Calonne M., Fontaine J., Debiane D., Laruelle F., Grandmougin-Ferjani A., Lounès-Hadj Sahraoui A. 2014. The arbuscular mycorrhizal Rhizophagus irregularis activates storage lipid biosynthesis to cope with the benzo[a]pyrene oxidative stress. Phytochemistry 97: 30-37.
  •  Calonne M., Fontaine J., Debiane D., Laruelle F., Grandmougin-Ferjani A., Lounès-Hadj Sahraoui A. 2014. Impact of anthracene on the arbuscular mycorrhizal fungus lipid metabolism. Botany 92 : 173-178.
  •  Tayeh, Ch., Randoux, B., Vincent, D., Bourdon, N. and Reignault, Ph. 2014. Exogenous trehalose induces defences in wheat before and during a biotic stress caused by powdery mildew. Phytopathology, 104, 293-305.
  •  Fayeulle A., Veignie e., Slomianny C., Dewailly E., Munch I.C., Rafin C. 2014. Energy- and cytoskeleton-dependent uptake of Benzo[a]pyrene by the telluric filamentous fungus Fusarium solani. Environmental Science and Pollution Research 21 : 3515-3523.